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sgrnas per gene  (Addgene inc)


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    Structured Review

    Addgene inc sgrnas per gene
    Sgrnas Per Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrnas+per+gene/Human+CRISPR+Metabolic+Gene+Knockout+Library+(Pooled+Library+%23110066)/bio_rxiv__64898__2026__01__24__701523-328-12-21
    Average 93 stars, based on 27 article reviews
    sgrnas per gene - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Genome Wide:

    Article Title: Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy.
    Article Snippet: Data were analysed by Seahorse Wave Desktop Software (Agilent). .. To generate lentivirus for screening, we used the genome-wide GeCKO v2.0 Human library52 in the lentiCRISPR v2 vector (Addgene, 1000000048), which contains six sgRNAs per gene (123,411 sgRNAs targeting 19,050 genes). ..

    Article Title: Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
    Article Snippet: Data were analysed by Seahorse Wave Desktop Software (Agilent). .. To generate lentivirus for screening, we used the genome-wide GeCKO v2.0 Human library in the lentiCRISPR v2 vector (Addgene, 1000000048), which contains six sgRNAs per gene (123,411 sgRNAs targeting 19,050 genes). ..

    Plasmid Preparation:

    Article Title: Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy.
    Article Snippet: Data were analysed by Seahorse Wave Desktop Software (Agilent). .. To generate lentivirus for screening, we used the genome-wide GeCKO v2.0 Human library52 in the lentiCRISPR v2 vector (Addgene, 1000000048), which contains six sgRNAs per gene (123,411 sgRNAs targeting 19,050 genes). ..

    Article Title: Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
    Article Snippet: Data were analysed by Seahorse Wave Desktop Software (Agilent). .. To generate lentivirus for screening, we used the genome-wide GeCKO v2.0 Human library in the lentiCRISPR v2 vector (Addgene, 1000000048), which contains six sgRNAs per gene (123,411 sgRNAs targeting 19,050 genes). ..

    CRISPR:

    Article Title: Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplification
    Article Snippet: For ARE-reporter assays, pREP-8xARE-GFP-SV40-BFP (Addgene, 134910) plasmid was transfected to cells with Lipofectamine 3000 (Thermo Fisher, L3000015). .. The metabolism-focused CRISPR knockout library , targeting 2,981 metabolic genes with 10 sgRNAs per gene (29,790 sgRNAs total), was obtained from Addgene (110066) and amplified following published protocols . ..

    Knock-Out:

    Article Title: Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplification
    Article Snippet: For ARE-reporter assays, pREP-8xARE-GFP-SV40-BFP (Addgene, 134910) plasmid was transfected to cells with Lipofectamine 3000 (Thermo Fisher, L3000015). .. The metabolism-focused CRISPR knockout library , targeting 2,981 metabolic genes with 10 sgRNAs per gene (29,790 sgRNAs total), was obtained from Addgene (110066) and amplified following published protocols . ..

    Amplification:

    Article Title: Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplification
    Article Snippet: For ARE-reporter assays, pREP-8xARE-GFP-SV40-BFP (Addgene, 134910) plasmid was transfected to cells with Lipofectamine 3000 (Thermo Fisher, L3000015). .. The metabolism-focused CRISPR knockout library , targeting 2,981 metabolic genes with 10 sgRNAs per gene (29,790 sgRNAs total), was obtained from Addgene (110066) and amplified following published protocols . ..



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